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Saccharomycves
cerevisiae
Saccharomyces cerevisiae Amino Acid Drop-Out Supplements
Saccharomyces cerevisiae Amino Acid Drop-Out Supplements Complete Supplement Mixture |
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La
miscela Complete Supplement Mixture (CSM) é una combinazione
di aminoacidi, vitamine ed altri elementi essenziali che favoriscono
una crescita vigorosa delle cellule di lievito sul mezzo colturale
addizionato di CSM. I terreni colturali Yeast Nitrogen Base without
amino acids addizionati di CSM si sono dimostrati essere una
eccellente combinazione per la coltivazione di quasi tutti i ceppi di
Saccharomyces cerevisiae e di supplementare gli auxotrofi con cui si
opera.
La
miscela CSM rappresenta anche la base per molte altre miscele “Drop
Out, utilizzate per selezionare i trasformanti e per le esigenze
nutrizionali degli auxotrofi. Ciascuna miscela CSM Drop Out contiene
tutti i componenti ad eccezione di uno fino a cinque elementi
nutritivi essenziali, cioè i supplementi “drop out”.
Alcune miscele CSM drop out sono anche disponibili con l’aggiunta
di extra aminoacidi e di altri componenti.
Le
formulazioni Complete Supplement Mixture sono disponibili in formati
da 10 e 100 g.
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Complete CSM Mixture
| Formato |
mg/l |
| Adenine |
10 |
| L-Arginine |
50 |
| L-Aspartic
acid |
80 |
| L-Histidine
HCl |
20 |
| L-Isoleucine |
50 |
| L-Leucine |
100 |
| L-Lysine
HCl |
50 |
| L-Methionine |
20 |
| L-Phenylalanine |
50 |
| L-Threonine |
100 |
| L-Tryptophan |
50 |
| L-Tyrosine |
50 |
| Uracil |
20 |
| Valine |
140 |
| Total |
790 |
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Conservare
tutte le miscele Drop out all’asciutto e a temperature ambientale
torna |
Complete CSM Mixture
| Formula |
mg/l |
10g |
100g |
| CSM |
790 |
DCS0011 |
DCS0019 |
| CSM,
+ 20 Ade |
800 |
DCS0021 |
DCS0029 |
| CSM,
+ 40 Ade |
820 |
DCS0031 |
DCS0039 |
torna
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Single Drop Outs
| Formula |
mg/l |
10g |
100g |
| CSM,
– Ade |
780 |
DCS0041 |
DCS0049 |
| CSM,
– Arg |
740 |
DCS0051 |
DCS0059 |
| CSM,
– Asp |
710 |
DCS0061 |
DCS0069 |
| CSM,
– His |
770 |
DCS0071 |
DCS0079 |
| CSM,
– Iso |
740 |
DCS0081 |
DCS0089 |
| CSM,
– Leu |
690 |
DCS0091 |
DCS0099 |
| CSM,
– Lys |
740 |
DCS0101 |
DCS0109 |
| CSM,
– Met |
770 |
DCS0111 |
DCS0119 |
| CSM,
– Phe |
740 |
DCS0121 |
DCS0129 |
| CSM,
– Thr |
690 |
DCS0131 |
DCS0139 |
| CSM,
– Trp |
740 |
DCS0141 |
DCS0149 |
| CSM,
– Tyr |
740 |
DCS0151 |
DCS0159 |
| CSM,
– Ura |
770 |
DCS0161 |
DCS0169 |
| CSM,
– Val |
650 |
DCS0171 |
DCS0179 |
| CSM,
– Arg, + 40 Ade |
770 |
DCS0181 |
DCS0189 |
| CSM,
– His, + 20 Ade |
780 |
DCS0191 |
DCS0199 |
| CSM,
– His, + 40 Ade |
800 |
DCS0201 |
DCS0209 |
| CSM,
– Leu, + 5 Ade |
685 |
DCS0211 |
DCS0219 |
| CSM,
– Leu, + 20 Ade |
700 |
DCS0221 |
DCS0229 |
| CSM,
– Leu, + 40 Ade |
720 |
DCS0231 |
DCS0239 |
| CSM,
– Lys, + 40 ade |
770 |
DCS0241 |
DCS0249 |
| CSM,
– Trp, + 5 Ade |
735 |
DCS0251 |
DCS0259 |
| CSM,
– Trp, + 20 Ade |
750 |
DCS0261 |
DCS0269 |
| CSM,
– Ura, + 20 Ade |
780 |
DCS0271 |
DCS0279 |
| CSM,
– Ura, + 40 Ade |
800 |
DCS0281 |
DCS0289 |
| CSM,
– Trp, + 10 Ala |
750 |
DCS0291 |
DCS0299 |
| CSM,
– Trp, + 25 Ala |
765 |
DCS0301 |
DCS0309 |
| CSM,
– Trp, + 50 Ala |
790 |
DCS0311 |
DCS0319 |
| CSM,
– His, + 10 Ala |
780 |
DCS0321 |
DCS0329 |
| CSM,
– His, + 25 Ala |
795 |
DCS0331 |
DCS0339 |
| CSM,
– His, + 50 Ala |
820 |
DCS0341 |
DCS0349 |
torna
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Double Drop Outs
| Formula |
mg/l |
10g |
100g |
| CSM,
– Ade, – Arg |
730 |
DCS0351 |
DCS0359 |
| CSM,
– Ade, – His |
760 |
DCS0361 |
DCS0369 |
| CSM,
– Ade, – Leu |
680 |
DCS0371 |
DCS0379 |
| CSM,
– Ade, – Lys |
730 |
DCS0381 |
DCS0389 |
| CSM,
– Ade, – Met |
760 |
DCS0391 |
DCS0399 |
| CSM,
– Ade, – Trp |
730 |
DCS0401 |
DCS0409 |
| CSM,
– Ade, – Ura |
760 |
DCS0411 |
DCS0419 |
| CSM,
– Arg, – His |
720 |
DCS0421 |
DCS0429 |
| CSM,
– Arg, – Leu |
640 |
DCS0431 |
DCS0439 |
| CSM,
– Arg, – Trp |
690 |
DCS0441 |
DCS0449 |
| CSM,
– Arg, – Ura |
720 |
DCS0451 |
DCS0459 |
| CSM,
– His, – Leu |
670 |
DCS0461 |
DCS0461 |
| CSM,
– His, – Lys |
720 |
DCS0471 |
DCS0479 |
| CSM,
– His, – Met |
750 |
DCS0481 |
DCS0489 |
| CSM,
– His, – Thr |
670 |
DCS0491 |
DCS0499 |
| CSM,
– His, – Trp |
720 |
DCS0501 |
DCS0509 |
| CSM,
– His, – Tyr |
720 |
DCS0511 |
DCS0519 |
| CSM,
– His, – Ura |
750 |
DCS0521 |
DCS0529 |
| CSM,
– Leu, – Lys |
640 |
DCS0531 |
DCS0539 |
| CSM,
– Leu, – Met |
670 |
DCS0541 |
DCS0549 |
| CSM,
– Leu, – Thr |
590 |
DCS0551 |
DCS0559 |
| CSM,
– Leu, – Trp |
640 |
DCS0561 |
DCS0569 |
| CSM,
– Leu, – Tyr |
640 |
DCS0571 |
DCS0579 |
| CSM,
– Leu, – Ura |
670 |
DCS0581 |
DCS0589 |
| CSM,
– Lys, – Met |
720 |
DCS0591 |
DCS0599 |
| CSM,
– Lys, – Thr |
640 |
DCS0601 |
DCS0609 |
| CSM,
– Lys, – Trp |
690 |
DCS0611 |
DCS0619 |
| CSM,
– Lys, – Tyr |
690 |
DCS0621 |
DCS0629 |
| CSM,
– Lys, – Ura |
720 |
DCS0631 |
DCS0639 |
| CSM,
– Met, – Trp |
720 |
DCS0641 |
DCS0649 |
| CSM,
– Met, – Ura |
750 |
DCS0651 |
DCS0659 |
| CSM,
– Thr, – Trp |
640 |
DCS0661 |
DCS0669 |
| CSM,
– Thr, – Ura |
670 |
DCS0671 |
DCS0679 |
| CSM,
– Trp, – Tyr |
690 |
DCS0681 |
DCS0689 |
| CSM,
– Trp, – Ura |
720 |
DCS0691 |
DCS0699 |
| CSM,
– Tyr, – Ura |
720 |
DCS0701 |
DCS0709 |
| CSM,
– His, – Leu, + 20 Ade |
680 |
DCS0711 |
DCS0719 |
| CSM,
– His, – Leu, + 40 Ade |
700 |
DCS0721 |
DCS0729 |
| CSM,
– His, – Trp, + 20 Ade |
730 |
DCS0731 |
DCS0739 |
| CSM,
– His, – Trp, + 40 Ade |
750 |
DCS0741 |
DCS0749 |
| CSM,
– His, – Ura, + 40 Ade |
780 |
DCS0751 |
DCS0759 |
| CSM,
– Leu, – Met, + 40 Ade |
700 |
DCS0761 |
DCS0769 |
| CSM,
– Leu, – Trp, + 20 Ade |
650 |
DCS0771 |
DCS0779 |
| CSM,
– Leu, – Trp, + 40 Ade |
670 |
DCS0781 |
DCS0789 |
| CSM,
– Leu, – Ura, + 40 Ade |
700 |
DCS0791 |
DCS0799 |
| CSM,
– Trp, – Ura, + 40 Ade |
750 |
DCS0801 |
DCS0809 |
| CSM,
– Leu, – Ura, + 30 Ser |
700 |
DCS0811 |
DCS0819 |
torna
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Triple CSM Mixture
| Formula |
mg/l |
10g |
100g |
| CSM,
– Ade, – Arg, – Lys |
680 |
DCS0821 |
DCS0829 |
| CSM,
– Ade, – His, – Leu |
660 |
DCS0831 |
DCS0839 |
| CSM,
– Ade, – His, – Trp |
710 |
DCS0841 |
DCS0849 |
| CSM,
– Ade, – His, – Ura |
740 |
DCS0851 |
DCS0859 |
| CSM,
– Ade, – Leu, – Met |
660 |
DCS0861 |
DCS0869 |
| CSM,
– Ade, – Leu, – Trp |
630 |
DCS0871 |
DCS0879 |
| CSM,
– Ade, – Leu, – Ura |
660 |
DCS0881 |
DCS0889 |
| CSM,
– Ade, – Met, – Trp |
710 |
DCS0891 |
DCS0899 |
| CSM,
– Ade, – Met, – Ura |
740 |
DCS0901 |
DCS0909 |
| CSM,
– Ade, – Trp, – Ura |
710 |
DCS0911 |
DCS0919 |
| CSM,
– Arg, – His, – Lys |
670 |
DCS0921 |
DCS0929 |
| CSM,
– Arg, – His, – Ura |
700 |
DCS0931 |
DCS0939 |
| CSM,
– Arg, – Leu, – Ura |
620 |
DCS0941 |
DCS0949 |
| CSM,
– Arg, – Trp, – Ura |
670 |
DCS0951 |
DCS0959 |
| CSM,
– His, – Leu, – Lys |
620 |
DCS0961 |
DCS0969 |
| CSM,
– His, – Leu, – Trp |
620 |
DCS0971 |
DCS0979 |
| CSM,
– His, – Leu, – Met |
650 |
DCS0981 |
DCS0989 |
| CSM,
– His, – Leu, – Ura |
650 |
DCS0991 |
DCS0999 |
| CSM,
– His, – Lys, – Trp |
670 |
DCS1001 |
DCS1009 |
| CSM,
– His, – Lys, – Ura |
700 |
DCS1011 |
DCS1019 |
| CSM,
– His, – Met, – Trp |
700 |
DCS1021 |
DCS1029 |
| CSM,
– His, – Met, – Ura |
730 |
DCS1031 |
DCS1039 |
| CSM,
– His, – Trp, – Val |
580 |
DCS1041 |
DCS1049 |
| CSM,
– His, – Trp, – Ura |
700 |
DCS1051 |
DCS1059 |
| CSM,
– Leu, – Lys, – Trp |
590 |
DCS1061 |
DCS1069 |
| CSM,
– Leu, – Lys, – Ura |
620 |
DCS1071 |
DCS1079 |
| CSM,
– Leu, – Met, – Trp |
620 |
DCS1081 |
DCS1089 |
| CSM,
– Leu, – Met, – Ura |
650 |
DCS1091 |
DCS1099 |
| CSM,
– Leu, – Trp, – Ura |
620 |
DCS1101 |
DCS1109 |
| CSM,
– Lys, – Trp, – Ura |
670 |
DCS1111 |
DCS1119 |
| CSM,
– Lys, – Tyr, – Ura |
670 |
DCS1121 |
DCS1129 |
| CSM,
– Met, – Trp, – Ura |
700 |
DCS1131 |
DCS1139 |
| CSM,
– Phe, – Trp, – Tyr |
640 |
DCS1141 |
DCS1149 |
| CSM,
– His, – Leu, – Trp, +20 Ade |
630 |
DCS1151 |
DCS1159 |
| CSM,
– His, – Leu, – Trp, +40 Ade |
650 |
DCS1161 |
DCS1169 |
| CSM,
– His, – Leu, – Ura, +20 Ser,+20
Pro, + 20 Cys |
710 |
DCS1171 |
DCS1179 |
| CSM,
– His, – Leu, – Trp, +20 Ser, +
20 Pro, + 20 Cys |
680 |
DCS1181 |
DCS1189 |
torna
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Quadruple CSM Mixture
| Formula |
mg/l |
10g |
100g |
| CSM,
– Ade, – Arg, – Leu, – Ura |
610 |
DCS1191 |
DCS1199 |
| CSM,
– Ade, – Arg, – Met, – Tyr |
660 |
DCS1201 |
DCS1209 |
| CSM,
– Ade, – His, – Leu, – Lys |
610 |
DCS1211 |
DCS1219 |
| CSM,
– Ade, – His, – Leu, – Trp |
610 |
DCS1221 |
DCS1229 |
| CSM,
– Ade, – His, – Leu, – Ura |
640 |
DCS1231 |
DCS1239 |
| CSM,
– Ade, – His, – Met, – Ura |
690 |
DCS1241 |
DCS1249 |
| CSM,
– Ade, – His, – Trp, – Ura |
690 |
DCS1251 |
DCS1259 |
| CSM,
– Ade, – Leu, – Lys, – Trp |
580 |
DCS1261 |
DCS1269 |
| CSM,
– Ade, – Leu, – Met, – Ura |
640 |
DCS1271 |
DCS1279 |
| CSM,
– Ade, – Leu, – Trp, – Ura |
610 |
DCS1281 |
DCS1289 |
| CSM,
– Ade, – Met, – Trp, – Ura |
690 |
DCS1291 |
DCS1299 |
| CSM,
– Arg, – His, – Lys, – Ura |
650 |
DCS1301 |
DCS1309 |
| CSM,
– Arg, – Leu, – Lys, – Ura |
570 |
DCS1311 |
DCS1319 |
| CSM,
– Arg, – Leu, – Trp, – Ura |
570 |
DCS1321 |
DCS1329 |
| CSM,
– Arg, – Lys, – Trp, – Ura |
620 |
DCS1331 |
DCS1339 |
| CSM,
– Asp, – His, – Leu, – Trp |
540 |
DCS1341 |
DCS1349 |
| CSM,
– His, – Leu, – Lys, – Trp |
570 |
DCS1351 |
DCS1359 |
| CSM,
– His, – Leu, – Met, – Trp |
600 |
DCS1361 |
DCS1369 |
| CSM,
– His, – Leu, – Lys, – Ura |
600 |
DCS1371 |
DCS1379 |
| CSM,
– His, – Leu, – Trp, – Ura |
600 |
DCS1381 |
DCS1389 |
| CSM,
– His, – Lys, – Trp, – Ura |
650 |
DCS1391 |
DCS1399 |
| CSM,
– His, – Met, – Trp, – Ura |
680 |
DCS1401 |
DCS1409 |
| CSM,
– Leu, – Lys, – Trp, – Ura |
570 |
DCS1411 |
DCS1419 |
| CSM,
– Leu, – Met, – Trp, –Ura |
600 |
DCS1421 |
DCS1429 |
| CSM,
– Arg, – His, – Leu, – Trp, +20 Cys,+
20 Pro, + 20 Ser, + 20 Ura |
630 |
DCS1431 |
DCS1439 |
| CSM,
– His, – Leu, – Trp, – Ura, +20 Cys,+
20 Pro, + 20 Ser |
660 |
DCS1441 |
DCS1449 |
torna
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Multiple CSM Mixture
| Formula |
mg/l |
10g |
100g |
| CSM,
– Ade, – Arg, – His, – Leu, –Met, –
Thr, – Trp, – Ura |
420 |
DCS1451 |
DCS1459 |
| CSM,
– Arg, – Asp, – Ile, – Met, –Phe, –
Thr,– Tyr, – Ura, – Val |
230 |
DCS1461 |
DCS1469 |
| CSM,
– Ade, – His, – Leu, – Lys, –Met, –
Thr, – Trp, – Tyr, – Ura |
370 |
DCS1471 |
DCS1479 |
| CSM,
– Ade, – His, – Leu, – Lys, –Trp, – Tyr,–
Ura |
490 |
DCS1481 |
DCS1489 |
| CSM,
– Ade, – His, – Leu, – Lys, –Trp, –
Ura |
540 |
DCS1491 |
DCS1499 |
| CSM,
– Ade, – His, – Leu, – Met, –Trp |
590 |
DCS1501 |
DCS1509 |
| CSM,
– Ade, – His, – Leu, – Met, –Trp, –
Ura |
570 |
DCS1511 |
DCS1519 |
| CSM,
– Ade, – His, – Leu, – Trp, – Ura |
590 |
DCS1521 |
DCS1529 |
| CSM,
– Ade, – His, – Lys, –Trp, – Ura |
640 |
DCS1531 |
DCS1539 |
| CSM,
– Ade, – Leu, – Lys, – Met, –Trp, –
Ura |
540 |
DCS1541 |
DCS1549 |
| CSM,
– Arg, – His, – Lys, –Trp, – Ura |
600 |
DCS1551 |
DCS1559 |
| CSM,
– His, – Leu, – Lys, – Met, –Thr, –
Trp, –Ura |
430 |
DCS1561 |
DCS1569 |
| CSM,
– His, – Leu, – Lys, –Trp, – Ura |
550 |
DCS1571 |
DCS1579 |
| CSM,
– His, – Leu, – Met, –Trp, – Ura |
580 |
DCS1581 |
DCS1589 |
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